Insight into response characteristics and inhibition mechanisms of anammox granular sludge to polyethylene terephthalate microplastics exposure
Xiantao Hong, Binxin Niu, Hongwei Sun, Xin Zhou
Abstract
Currently, in-depth understanding of response characteristics and mechanisms of anammox process under microplastics (MPs) stress remains quite limited. This study investigated the influence of 0.1-1.0 g/L polyethylene terephthalate (PET) on anammox granular sludge (AnGS). Compared with the control, 0.1-0.2 g/L PET did not significantly affect the anammox efficiency, while the anammox activity decreased by 16.2% at 1.0 g/L PET. Integrity coefficient and transmission electron microscopy analysis demonstrated that the strength and structural stability of the AnGS became weaken following exposure to 1.0 g/L PET. With the PET increasing, the abundance of anammox genera and genes related to energy metabolism and cofactors and vitamins metabolism decreased. The reactive oxygen species generated in the interaction between microbial cells and PET resulting in cellular oxidative stress was responsible for inhibiting anammox. These findings give novel insights into the anammox behavior in biological nitrogen removal systems treating PET-loaded nitrogenous wastewater.