Single‐cell transcriptomics of suprachiasmatic nuclei reveal a Prokineticin‐driven circadian network

Abstract

Circadian rhythms in mammals are governed by the hypothalamic suprachiasmatic nucleus (SCN), in which 20,000 clock cells are connected together into a powerful time‐keeping network. In the absence of network‐level cellular interactions, the SCN fails as a clock. The topology and specific roles of its distinct cell populations (nodes) that direct network functions are, however, not understood. To characterise its component cells and network structure, we conducted single‐cell sequencing of SCN organotypic slices and identified eleven distinct neuronal sub‐populations across circadian day and night. We defined neuropeptidergic signalling axes between these nodes, and built neuropeptide‐specific network topologies. This revealed their temporal plasticity, being up‐regulated in circadian day. Through intersectional genetics and real‐time imaging, we interrogated the contribution of the Prok2‐ProkR2 neuropeptidergic axis to network‐wide time‐keeping. We showed that Prok2‐ProkR2 signalling acts as a key regulator of SCN period and rhythmicity and contributes to defining the network‐level properties that underpin robust circadian co‐ordination. These results highlight the diverse and distinct contributions of neuropeptide‐modulated communication of temporal information across the SCN. Single‐cell transcriptomics reveal eleven distinct neuronal subpopulations of the suprachiasmatic nucleus (SCN) circadian network. Neuropeptidergic signalling between these neuronal subtypes is circadian‐regulated with Prokineticin2 signaling acting as a key determinant of SCN period and rhythmicity. Eleven distinct neuronal sub‐populations constitute the central circadian clock in the mammalian hypothalamus, and integrate temporal information via diverse and distinct contributions of neuropeptide‐modulated communication.

Topics & Concepts