One-pot synthesis of trypsin-based magnetic metal–organic frameworks for highly efficient proteolysis
Chao Zhong, Zhixian Lei, Huan Huang, Mingyue Zhang, Zongwei Cai, Zian Lin
Abstract
) which made the operation and recycling easy and convenient. In addition, iron oxide@ZIF-8@trypsin could be applied as an immobilized enzyme microreactor (IMER) to rapidly and efficiently digest proteins and complex human serum samples with satisfactory results, showing great promise for application in proteomic analysis.
Topics & Concepts
ProteolysisTrypsinMetal-organic frameworkMaterials scienceMetalNanotechnologyCombinatorial chemistryOrganic chemistryChemistryMetallurgyEnzymeAdsorptionMetal-Organic Frameworks: Synthesis and ApplicationsAdvanced Nanomaterials in CatalysisMolecular Sensors and Ion Detection