Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis
Lei Huang, Xiaohui Li, Weiwei Zhang, Nolan Ung, Nana Liu, Xianglin Yin, Yong Li, Robert E. McEwan, Brian P. Dilkes, Mingji Dai, Glenn R. Hicks, Natasha V. Raikhel, Christopher J. Staiger, Chunhua Zhang
Abstract
). Chemical genetic analysis revealed important amino acids that potentially participate in the catalytic activity of plant CESA6, in addition to previously identified conserved motifs across kingdoms. Using high spatiotemporal resolution live cell imaging, we found that inhibiting the catalytic activity of CESA6 by ES20 treatment reduced the efficiency of CSC transport to the plasma membrane. Our results demonstrate that ES20 is a chemical inhibitor of CESA activity and trafficking that represents a powerful tool for studying cellulose synthesis in plants.