Lsr2 and Its Novel Paralogue Mediate the Adjustment of Mycobacterium smegmatis to Unfavorable Environmental Conditions
Marta Kołodziej, Tomasz Łebkowski, Przemysław Płociński, Joanna Hołówka, Mariola Paściak, Bartosz Wojtaś, Katarzyna Bury, Igor Konieczny, Jarosław Dziadek, Jolanta Zakrzewska-Czerwińska
Abstract
, MSMEG_1060. We found that Lsr2 plays a role in transcriptional regulation, mainly by repressing gene expression via DNA loop formation and/or DNA-coating mechanisms. Intriguingly, the number of Lsr2-mediated genes was found to increase under hypoxia. Compared to Lsr2, MSMEG_1060 exhibits a different DNA binding specificity and chromosomal localization. Since tuberculosis remains a serious worldwide health problem, studies on stress response-mediating agents, such as Lsr2, may contribute to the development of novel antituberculosis drugs.
Topics & Concepts
Mycobacterium smegmatisNucleoidBiologyGeneChromatinGeneticsChromatin immunoprecipitationDNARepressorTranscription (linguistics)Mycobacterium tuberculosisCell biologyPromoterTranscription factorGene expressionEscherichia coliTuberculosisMedicinePhilosophyLinguisticsPathologyMycobacterium research and diagnosisTuberculosis Research and EpidemiologyInfectious Diseases and Mycology