Molecular identification of nematodes using polymerase chain reaction (PCR).
Sergei A. Subbotin
Abstract
<title>Abstract</title> Molecular diagnostics are a vital component of the management of economically important pests, including plant-parasitic nematodes. Various molecular techniques for diagnostics have been introduced to nematology during last decades, but the most popular is Polymerase Chain Reaction (PCR) based. This chapter presents procedures for DNA extraction, PCR techniques, cloning and DNA sequencing of nematodes.
Topics & Concepts
Polymerase chain reactionIdentification (biology)Polymerase chain reaction optimizationBiologyComputational biologyMolecular biologyMultiplex polymerase chain reactionChemistryGeneticsGeneBotanyNematode management and characterization studies