Fluorescence imaging of hypochlorous acid and peroxynitrite<i>in vitro</i>and<i>in vivo</i>with emission wavelength beyond 750 nm
Linfang Wang, Jing Liu, Shengwei Zhao, Hong‐Xing Zhang, Yuanqiang Sun, Aihua Wei, Wei Guo
Abstract
A series of near-infrared and photostable Si-oxazine fluorescent dyes was synthesized using a simple three-step procedure, and one of their reduced products, i.e. hydro-Si-oxazine HSiO3, has been utilized to sensitively detect hypochlorous acid and peroxynitrite generation by phagocytes in inflamed and pulmonary fibrosis diseased mice with emission wavelength beyond 750 nm.
Topics & Concepts
PeroxynitriteHypochlorous acidFluorescenceIn vivoChemistryPhotochemistryIn vitroBiophysicsAggregation-induced emissionNanotechnologyBiochemistryMaterials scienceSuperoxideOpticsPhysicsBiologyBiotechnologyEnzymeNeutrophil, Myeloperoxidase and Oxidative MechanismsNitric Oxide and Endothelin EffectsMolecular Sensors and Ion Detection