Citral Protects Human Endothelial Cells Against Hydrogen Peroxide-induced Oxidative Stress
Leila Safaeian, Seyed Ebrahim Sajjadi, Hossein Montazeri, Farzaneh Ohadi, Shaghayegh Haghjooy Javanmard
Abstract
OBJECTIVES: )-induced oxidative stress. MATERIALS AND METHODS: (0.5 mM, 2 h). Cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The hydroperoxide concentrations and ferric reducing ability of plasma (FRAP) were measured in intra- and extracellular fluids. RESULTS: -induced cytotoxicity. It reduced intracellular hydroperoxide levels at the concentrations of 5 and 10 μg/mL and extracellular hydroperoxide levels at the concentrations of 2.5-10 μg/mL. Pretreatment with citral significantly increased the FRAP value in intra- and extracellular fluids at the concentration range of 1.25-10 μg/mL. CONCLUSION: in human endothelial cells. However, more studies in this area are needed to assess its clinical value for prevention and treatment of cardiovascular diseases.