A CRISPR Interference Platform for Selective Downregulation of Gene Expression in Borrelia burgdorferi
Constantin N. Takacs, Molly Scott, Yunjie Chang, Zachary A. Kloos, Irnov Irnov, Patricia A. Rosa, Jun Liu, Christine Jacobs‐Wagner
Abstract
Gene function studies are facilitated by the availability of rapid and easy-to-use genetic tools. Homologous recombination-based methods traditionally used to genetically investigate gene function remain cumbersome to perform in B. burgdorferi , as they often are relatively inefficient.
Topics & Concepts
Borrelia burgdorferiBiologyMreBCRISPRCell biologyCRISPR interferencePeriplasmic spaceFlagellinGeneticsMicrobiologyGeneMechanosensitive channelsCas9Escherichia coliIon channelReceptorAntibodyVector-borne infectious diseasesInsect symbiosis and bacterial influencesCRISPR and Genetic Engineering