A Nucleocapsid-based Transcomplementation Cell Culture System of SARS-CoV-2 to Recapitulate the Complete Viral Life Cycle
Yanying Yu, Xiaohui Ju, Qiang Ding
Abstract
cells as a packaging cell line to further enhance the security of this cell culture model. Altogether, this system provides for a safe and convenient method to produce trVLPs in BSL-2 laboratories. These trVLPs can be modified to incorporate desired mutations, permitting high-throughput screening of antiviral compounds and evaluation of neutralizing antibodies. This protocol describes the details of the trVLP cell culture model to make SARS-CoV-2 research more readily accessible.
Topics & Concepts
Cell cultureBiologyViral life cycleVirologyCell biologyBiosafetyComputational biologyVirusRNA splicingGeneReporter geneCellTransfectionGreen fluorescent proteinReverse geneticsEctopic expressionElectroporationCell cycleViral vectorGene expressionCoronavirusHEK 293 cellsGeneticsViral replicationGenetically modified organismCoronavirus disease 2019 (COVID-19)PlasmidViral cultureHuman cellMolecular biologySARS-CoV-2 and COVID-19 ResearchViral Infectious Diseases and Gene Expression in InsectsTransgenic Plants and Applications