Molecular characterization and pathogenicity of an infectious cDNA clone of tomato brown rugose fruit virus
Ziyue Ma, Hui Zhang, Ming Ding, Zhongkai Zhang, Xiuling Yang, Xueping Zhou
Abstract
Abstract Tomato brown rugose fruit virus (ToBRFV) is a new member of the genus Tobamovirus , and has the potential to affect the production and marketability of tomatoes and peppers. In this study, we sequenced and analyzed the complete genome of ToBRFV isolates from tomato plants showing mosaic and mottling symptoms in Yunnan Province of China. We constructed a full-length infectious cDNA clone of ToBRFV, which could induce systemic infection with typical symptoms in tomato, Nicotiana benthamiana , and N. tabacum cv. Samsun nn plants through Agrobacterium -mediated inoculation. Further experimental evidence demonstrated that the rod-shaped virions accumulating in agroinfiltrated plants are sap-transmissible. This is the first report on the construction of a biologically active, full-length infectious cDNA clone of ToBRFV. The system developed herein will facilitate further research on functions of ToBRFV-encoded proteins and plant-ToBRFV interactions through reverse genetic approaches.