Dioxygenase JID1 mediates the modification of OPDA to regulate jasmonate homeostasis
Yi Rong, Ran Du, Jiaojiao Wang, Jijun Yan, Jinfang Chu, Jianbin Yan, Xiaoyi Shan, Daoxin Xie, Xiaoyi Shan, Daoxin Xie
Abstract
The phytohormone jasmonate (JA), including jasmonic acid and its oxylipin derivatives, is essential for plant resistance against various stresses 1 , 2 . The biosynthetic pathway of JA is initiated from α-linolenic acid (18:3) that is converted to 12-oxo-phytodienoic acid (OPDA) by 13-Lipoxygenase (LOX), Allene Oxide Synthase (AOS), and Allene Oxide Cyclase (AOC), which is then reduced to OPC-8:0 by OPDA Reductase3 (OPR3) and activated by OPC-8:0 CoA Ligase1 (OPCL1) followed by three cycles of β-oxidation catalyzed by acyl-CoA Oxidase (ACX), Multifunctional Protein (MFP), and 3-ketoacyl-CoA Thiolase (KAT) to yield jasmonic acid. Jasmonic acid is further catalyzed by Jasmonate Resistant1 (JAR1) to generate jasmonoyl-l-isoleucine (JA-Ile) 1 . Excess jasmonic acid and JA-Ile are hydroxylated by Jasmonate-Induced Oxygenases (JOX1, JOX2, JOX3, and JOX4) that belong to 2-oxoglutarate/Fe(II)-dependent dioxygenase (2OGD) superfamily, and cytochrome P450 enzymes (CYP94B3, CYP94B1, and CYP94C1) for the regulation of JA homeostasis, respectively 3 , 4 , thereby driving the growth-defense tradeoff in plants.