Effect of <i>Bacillus subtilis</i>, <i>Enterococcus faecium,</i> and <i>Enterococcus faecalis </i>supernatants on serotonin transporter expression in cells and tissues
Yi‐Ming Chen, Ying Li, Xin Wang, Zelan Wang, Junjie Hou, Shuai Su, Weilong Zhong, Xin Xu, Jie Zhang, Bangmao Wang, Yuming Wang
Abstract
BACKGROUND: ) are probiotics that are widely used in the clinical treatment of irritable bowel syndrome (IBS). Whether the supernatants of these three probiotics can improve gastrointestinal sensation and movement by regulating the serotonin transporter (SERT) expression needs to be clarified. AIM: . METHODS: Caco-2 and HT-29 cells were stimulated with probiotic culture supernatants for 12 and 24 h, respectively. A male Sprague-Dawley rat model of post-infectious irritable bowel syndrome (PI-IBS) was established and the rats were treated with phosphate-buffered saline (group A) and three probiotics culture supernatants (groups B, C, and D) for 4 wk. The levels of SERT were detected by quantitative PCR and western blotting. RESULTS: 0.05). CONCLUSION: can upregulate SERT expression in intestinal epithelial cells and the intestinal tissues in the rat model of PI-IBS.