Moderate-Intensity Exercise and Musical Co-Treatment Decreased the Circulating Level of Betatrophin
Hendra Susanto, Ahmad Taufiq, Sugiharto, Desiana Merawati, Khumaira Marsyidah Badu, Jerry Dwi Trijoyo Purnomo, Adeodatus Yuda Handaya
Abstract
Introduction . In general, the significant contribution of lack of physical activity is strongly correlated with lipid metabolism and metabolic disorder. Hitherto, betatrophin is a potential hormone that regulates the lipid profile in the body circulation-associated triglyceride level. This study was designed to evaluate the alteration of betatrophin levels in subject-onset hypertriglyceridemia with exercise intervention co-treated with music. Materials and Methods . A total of 60 nonprofessional athletes were enrolled in this study and given moderate-intensity exercise (MIE) combined with middle rhythm musical co-treatment. The ELISA method was applied to quantify the serum level of betatrophin in all samples. The statistical analysis was performed by applying the Kolmogorov–Smirnov normality test, one-way ANOVA, and parametric linear correlation and regression. Results . Interestingly, our data show that MIE decreased the circulating level of betatrophin combined with music (12.47 ± 0.40 ng/mL) compared with that without musical co-treatment (20.81 ± 1.16 ng/mL) and high-intensity exercise (26.91 ± 2.23 ng/mL). The plasma level of betatrophin was positively correlated with triglycerides ( r = 0.316, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.05</mml:mn></mml:math>), systolic blood pressure ( r = 0.428, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.01</mml:mn></mml:math>), HDL ( r = 0.366, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.05</mml:mn></mml:math>), energy expenditure ( r = 0.586, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.001</mml:mn></mml:math>), PGC-1 α ( r = 0.573, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.001</mml:mn></mml:math>), and irisin ( r = 0.863, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M6"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.001</mml:mn></mml:math>). By contrast, the plasma level of betatrophin was negatively associated with age ( r = −0.298, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M7"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.05</mml:mn></mml:math>) and LDL cholesterol ( r = −0.372, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M8"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.05</mml:mn></mml:math>). Importantly, betatrophin is a significant predictor for energy expenditure (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M9"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.001</mml:mn></mml:math>) and plasma triglyceride levels (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M10"><mml:mi>p</mml:mi><mml:mo>≤</mml:mo><mml:mn>0.05</mml:mn></mml:math>). Conclusions . Our data demonstrate that betatrophin levels decreased the post-MIE and musical therapeutical combination. Therefore, betatrophin may provide a benefit as the potential biomarker of physiological performance-associated physical training.