Cascaded filter deterministic lateral displacement microchips for isolation and molecular analysis of circulating tumor cells and fusion cells
Zongbin Liu, Yuqing Huang, Wenli Liang, Jing Bai, Hongtao Feng, Zhihao Fang, Geng Tian, Yanjuan Zhu, Haibo Zhang, Yuanxiang Wang, Aixue Liu, Yan Chen
Abstract
). Samples from lung cancer patients were analyzed using the CFD-Chip, CTCs and tumor cell-leukocyte fusion cells were efficiently collected, and changes in CTC levels were used for treatment response monitoring. The CFD-Chip platform isolated CTCs with good viability, enabling direct downstream analysis with single-cell RNA sequencing. Transcriptome analysis of enriched CTCs identified new subtypes of CTCs such as tumor cell-leukocyte fusion cells, providing insights into cancer diagnostics and therapeutics.
Topics & Concepts
Displacement (psychology)Isolation (microbiology)Filter (signal processing)Circulating tumor cellFusionMaterials scienceNanotechnologyEngineeringComputer scienceBiological systemBiologyBioinformaticsElectrical engineeringGeneticsCancerPsychotherapistLinguisticsMetastasisPsychologyPhilosophyMicrofluidic and Bio-sensing TechnologiesAdvanced MEMS and NEMS TechnologiesMicrofluidic and Capillary Electrophoresis Applications