Transferrin receptor targeting by de novo sheet extension
Danny D. Sahtoe, Adrian Coscia, Nur Mustafaoğlu, L. M. Miller, Daniel Olal, Ivan Vulovic, Ta-Yi Yu, Inna Goreshnik, Yu‐Ru Lin, Lars E. Clark, Florian Büsch, Lance Stewart, Vicki H. Wysocki, Donald E. Ingber, Jonathan Abraham, David Baker
Abstract
Significance The de novo design of proteins that bind natural target proteins is useful for a variety of biomedical and biotechnological applications. We describe a design strategy to target proteins containing an exposed beta edge strand. We use the approach to design binders to the human transferrin receptor which shuttles back and forth across the blood–brain barrier. Such binders could be useful for the delivery of therapeutics into the brain.
Topics & Concepts
TransferrinTransferrin receptorBlood–brain barrierReceptorComputational biologyBiologyCell biologyNeuroscienceBiochemistryCentral nervous systemMonoclonal and Polyclonal Antibodies ResearchPeptidase Inhibition and AnalysisGlycosylation and Glycoproteins Research