An integrated analysis of human myeloid cells identifies gaps in in vitro models of in vivo biology
Nadia Rajab, Paul W. Angel, Yidi Deng, Jennifer Gu, Vanta Jameson, Mariola Kurowska‐Stolarska, Simon Milling, Chris M. Pacheco, Matt Rutar, Andrew L. Laslett, Kim‐Anh Lê Cao, Jarny Choi, Christine A. Wells
Abstract
The Stemformatics myeloid atlas is an integrated transcriptome atlas of human macrophages and dendritic cells that systematically compares freshly isolated tissue-resident, cultured, and pluripotent stem cell-derived myeloid cells. Three classes of tissue-resident macrophage were identified: Kupffer cells and microglia; monocyte-associated; and tumor-associated macrophages. Culture had a major impact on all primary cell phenotypes. Pluripotent stem cell-derived macrophages were characterized by atypical expression of collagen and a highly efferocytotic phenotype. Myeloid subsets, and phenotypes associated with derivation, were reproducible across experimental series including data projected from single-cell studies, demonstrating that the atlas provides a robust reference for myeloid phenotypes. Implementation in Stemformatics.org allows users to visualize patterns of sample grouping or gene expression for user-selected conditions and supports temporary upload of your own microarray or RNA sequencing samples, including single-cell data, to benchmark against the atlas.