Major phenolic compounds, antioxidant and antibacterial activities of Anthemis praecox Link aerial parts
Saber Belhaoues, Sandra Amri, Mourad Bensouilah
Abstract
Anthemis praecox Link is commonly used in Algerian's rural communities folk medicine for the treatment of infectious and gastrointestinal disorders. This study was designed to determine a phenolic profile, antioxidant and antibacterial potential of (dichloromethane, ethyl acetate, n-butanol and aqueous) fractions obtained from the aerial parts of this plant. Spectrophotometric and RP-HPLC analyses were carried out to determinate phenolic profile of each fraction. Antioxidant activity was determined by diphenyl 2-pycril hydrazil (DPPH), 2, 2′-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS), and β carotene linoleate bleaching (BCB) assays. Antibacterial activity was determined by the agar diffusion method. The results showed that ethyl acetate fraction contained the highest amount of phenolic compounds. Naringin (68,7 ± 16,8 µg/mg), tannic acid (47,64 ± 4,2 µg/mg), and quercetin (44,80 ± 2,2 µg/mg) were found as the major phenolic compounds as judged by RP-HPLC. Also ethyl acetate fraction exhibited the highest antioxidant activity in DPPH and ABTS assays with an IC50 value of 0,11 and 0,56 mg/mL respectively, while the butanolic fraction demonstrated the highest antioxidant activity in BCB assay (PI = 58,65%). In addition, ethyl acetate fraction showed promising antibacterial activity, with good efficiency against Gram bacteria. Staphylococcus aureus (19 mm at 10 mg/disk) was found as the most sensitive bacteria.