Apoptosis-inducing activity of a fluorescent barrel-rosette M<sup>+</sup>/Cl<sup>−</sup> channel
Javid Ahmad Malla, Rintu M. Umesh, Amal Vijay, Arnab Mukherjee, Mayurika Lahiri, Pinaki Talukdar
Abstract
in the preequilibrated POPC lipid bilayer with water molecules illustrated various aspects of channel formation and ion permeation. Cell viability assay with the designed compounds indicated that cell death is being induced by the individual compounds which follow the order of their ion transport activity and chloride and cations play roles in cell death. The inherent fluorescence of the most active transporter was helpful to monitor its permeation in cells by confocal microscopy. The apoptosis-inducing activity upon perturbation of intracellular ionic homeostasis was established by monitoring mitochondrial membrane depolarization, generation of reactive oxygen species, cytochrome c release, activation of the caspase 9 pathway, and finally the uptake of the propidium iodide dye in the treated MCF7 cells.