Human Amniotic Fluid Mesenchymal Stem Cell‐Derived Exosomes Inhibit Apoptosis in Ovarian Granulosa Cell via miR‐369‐3p/YAF2/PDCD5/p53 Pathway
Zixiang Geng, Haiyang Chen, Gang Zou, Long Yuan, Peng Liu, Bingrong Li, Kaiyong Zhang, Fangyuan Jing, Xiaoli Nie, Te Liu, Bimeng Zhang
Abstract
Human amniotic fluid stem cell‐derived exosome (HuAFSC‐exosome) transplantation is considered a promising treatment for premature ovarian failure (POF). However, its mechanism remains unclear. In this study, exosomes were isolated and enriched from HuAFSC subsets of CD44+/CD105+, and the exosomes were transplanted into a POF model in vitro and in vivo . Our results confirmed that the exosomes produced by CD44+/CD105+ HuAFSCs could achieve therapeutic effects in a mouse POF model. Our research also showed that CD44+/CD105+ HuAFSC‐exosomes carrying miR‐369‐3p could specifically downregulate the expression of YAF2, inhibit the stability of PDCD5/p53, and reduce the apoptosis of ovarian granulosa cells (OGCs), thereby exerting therapeutic effects on POF. Knowledge of these mechanisms demonstrates that miRNAs carried by CD44+/CD105+ HuAFSC‐exosomes are critical to the therapy of POF. This will be useful for the clinical application of stem cells.