A hemicyanine-based fluorescent probe for ratiometric detection of ClO- and turn-on detection of viscosity and its imaging application in mitochondria of living cells and zebrafish
Si-Lin Xu, Fangfang Guo, Zhi-Hong Xu, Yuan Wang, Tony D. James
Abstract
A water-soluble fluorescent probe 1 based on chromene-hemicyanine has been developed, that exhibits ratiometric fluorescent response toward hypochlorite (ClO-) with the LOD (0.082 μM), and “turn-on” response to viscosity changes. Due to low cytotoxicity and good biocompatibility, probe 1 used to monitor hypochlorous acid and viscosity in mitochondria of living cells and zebrafish.
Topics & Concepts
Hypochlorous acidZebrafishFluorescenceBiocompatibilityHypochloriteCytotoxicityViscosityTurn (biochemistry)ChemistryMitochondrionBiophysicsPhotochemistryBiochemistryMaterials scienceOrganic chemistryBiologyIn vitroComposite materialGenePhysicsQuantum mechanicsMolecular Sensors and Ion DetectionAnalytical Chemistry and SensorsNitric Oxide and Endothelin Effects