Sanger Tree of Life HMW DNA Extraction: Manual Nucleated Blood Nanobind® v1
Amy Denton, graeme oatley, Pacific Biosciences, Caroline Howard
Abstract
This protocol describes the manual extraction of HMW DNA from nucleated blood samples intended for long-read sequencing using the Nanobind® tissue kit and following the ‘Extracting HMW DNA from nucleated blood using Nanobind® kits’ procedure from PacBio. This process is effective for any species with nucleated blood within the Chordate group, covered by the Tree of Life Programme, however difficulties can arise with samples where preservation has not been optimal. The output of this protocol is high quality and quantity HMW DNA, which can be directed towards the HMW DNA Fragmentation: Diagenode Megaruptor®3 for LI PacBio protocol. Acronyms: HMW: high-molecular weight LI: low input