Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice
Simon Heß, Helmut Wratil, Peter Kloppenburg
Abstract
Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are disturbed, significantly altering the electrophysiological properties of the measured neurons. Perforated-patch clamp recordings circumvent this issue, allowing long-term electrophysiological recordings with minimized impairment of the intracellular milieu. Based on previous studies, we describe a superstition-free protocol that can be used to routinely perform perforated patch clamp recordings for current and voltage measurements.
Topics & Concepts
ElectrophysiologyPatch clampCurrent clampNeuroscienceVoltage clampSlice preparationClampIntracellularEx vivoBiologyIn vivoChemistryComputer scienceCell biologyComputer visionBiotechnologyClampingNeural dynamics and brain functionNeuroscience and Neural EngineeringNeuroscience and Neuropharmacology Research