Refining the reproducibility of a murine model of stress-induced reversible cardiomyopathy
Tomohiro Hayashi, Sajal K. Tiwary, Kenji Rowel Q. Lim, Cibele Rocha‐Resende, Attila Kovács, Carla J. Weinheimer, Douglas L. Mann
Abstract
The present study highlights the importance of sexual dimorphism with respect to isoproterenol injury, as well as the importance of animal handling and welfare to obtain reproducible results from investigator to investigator. Based on serial observations of animal recovery (locomotor activity and grooming behavior), troponin I release, and inflammation, we identified that the method used to restrain the mice for the intraperitoneal injection was the single greatest source of variability in this model.
Topics & Concepts
CardiomyopathyMedicineInternal medicineCardiologyAnimal modelReproducibilitySubcutaneous injectionHeart failureChemistryChromatographyTakotsubo Cardiomyopathy and Associated PhenomenaCardiac Structural Anomalies and RepairCardiac Arrest and Resuscitation