Cross-comparison of microbiota in the oropharynx, hypopharyngeal squamous cell carcinoma and their adjacent tissues through quantitative microbiome profiling
Hui‐Ching Lau, Yujie Shen, Huiying Huang, Xiaohui Yuan, Mengyou Ji, Hongli Gong, Chi‐Yao Hsueh, Liang Zhou
Abstract
Aims: To clarify the absolute abundance of microbial communities on hypopharyngeal squamous cell carcinoma and their correlation to those in the oropharynx. Methods: Clinical data, swabs, and tissue samples from 27 HPSCC patients were collected in this study and divided into three sampling groups: 19 oropharyngeal mucosa (OPM), 27 hypopharyngeal carcinomas tissues (HC), and 26 corresponding adjacent tissues (AT). Relative microbiome profiling (RMP), and quantitative microbiome profiling (QMP) of 16S rRNA amplicon sequencing were used for analysis. Results: were found to be enhanced in higher T classifications (T3-4) and advanced stages (Ⅳ). Conclusions: were deemed asrisk factors of advanced HPSCC.