Genome Mining-Driven Discovery of 5-Methylorsellinate-Derived Meroterpenoids from <i>Aspergillus funiculosus</i>

Abstract

Heterologous expression of a cryptic gene cluster in the fungus Aspergillus funiculosus CBS 116.56 led to the discovery of four new meroterpenoids, funiculolides A–D (1–4), derived from the aromatic polyketide 5-methylorsellinic acid (5-MOA). Intriguingly, funiculolide D (4), the apparent end product of the pathway, harbors an unusual spirocyclopentanone moiety, which is synthesized by the oxidative rearrangement catalyzed by the ferrous iron and α-ketoglutarate-dependent dioxygenase FncG.

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