Integrated biosensors for monitoring microphysiological systems
Lei Mou, Kalpana Mandal, Marvin Mecwan, Ana López Hernández, Surjendu Maity, Saurabh Sharma, Rondinelli Donizetti Herculano, Satoru Kawakita, Vadim Jucaud, Mehmet R. Dokmeci, Ali Khademhosseini
Abstract
modeling is the accurate phenotypical and functional characterization of the modeled organ. However, most conventional characterization methods are invasive and destructive and do not allow continuous monitoring of the cells in culture. On the other hand, microfluidic biosensors enable in-line, real-time sensing of target molecules with an excellent limit of detection and in a non-invasive manner, thereby effectively overcoming the limitation of the traditional techniques. Consequently, microfluidic biosensors have been increasingly integrated into MPSs and used for in-line target detection. This review discusses the state-of-the-art microfluidic biosensors by providing specific examples, detailing their main advantages in monitoring MPSs, and highlighting current developments in this field. Finally, we describe the remaining challenges and potential future developments to advance the current state-of-the-art in integrated microfluidic biosensors.