Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFNγ+LPS), M(IL-4) and M(IL-10) phenotypes
Euan W. Baxter, A. Graham, Nikki Alexandra Re, Ian Carr, James Robinson, Sarah Mackie, Ann W Morgan
Topics & Concepts
THP1 cell lineCD163MonocyteCell biologyMacrophage polarizationMacrophageCytokineMannose receptorReceptorBiologyPhenotypeCell cultureImmunologyIn vitroChemistryMolecular biologyGeneBiochemistryGeneticsImmune cells in cancerImmune Cell Function and InteractionImmunotherapy and Immune Responses