Preparation of gelatin/Ag NPs under ultrasound condition: A potent and green bio-nanocomposite for the treatment of pleomorphic hepatocellular carcinoma, morris hepatoma, and novikoff hepatoma
Zukuan Chang, Bikash Karmakar, Huilin Lu, Xuelei Lou, Shadi Alkhayyat, Gomaa Mostafa‐Hedeab, Gaber El‐Saber Batiha, Attalla F. El‐kott, Fahmy G. Elsaid, Mohamed A. Al‐Kahtani, Mutasem Z. Bani‐Fwaz
Abstract
Regarding applicative, facile, green chemical research, a bio-inspired approach is being reported for the sonochemical synthesis of Ag nanoparticles by gelatin as a natural reducing/stabilizing and solid support agent without using any toxic and harmful reagent. The biosynthesized Ag [email protected] bionanocomposite were characterized by advanced physicochemical techniques like ultraviolet–visible (UV–Vis), Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), High-Resolution Transmission Electron Microscopy (HR-TEM), Energy Dispersive X-ray spectroscopy (EDX), and X-ray Diffraction (XRD) study. It has been established that gelatin-stabilized silver nanoparticles have a spherical shape with a mean diameter from ∼5–10 nm. To survey the antioxidant potentials of Ag [email protected], one of the common antioxidant techniques i.e., DPPH was used. The results of DPPH test proved excellent antioxidant properties of Ag [email protected] in comparison to the positive control used i.e., butylated hydroxytoluene. The IC50 of Ag [email protected] was 250 µg/mL in the antioxidant test. Determination of anti-liver cancer effects of Ag [email protected] was carried out by the MTT assay and against pleomorphic hepatocellular carcinoma (SNU-387), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cell lines. The results of MTT assay confirmed removing SNU-387, McA-RH7777, and N1-S1 Fudr cell lines after treating with low concentrations of Ag [email protected] The IC50 of the Ag [email protected] was 192, 144, and 228 µg/mL against SNU-387, McA-RH7777, and N1-S1 Fudr cell lines, respectively. The best result of anti-human liver cancer effects of Ag [email protected] against the above cell lines was seen in the case of the N1-S1 Fudr cell line. According to the above results, significant anti-liver cancer effects of Ag [email protected] is confirmed.