Pressure-Jump Kinetics of Liquid–Liquid Phase Separation: Comparison of Two Different Condensed Phases of the RNA-Binding Protein, Fused in Sarcoma
Ryo Kitahara, Ryota Yamazaki, Fumika Ide, Shujie Li, Yutaro Shiramasa, Naoya Sasahara, Takuya Yoshizawa
Abstract
= 1.4). The HP-LLPS vanishing rate was ∼20-fold slower than that of LP-LLPS. This difference in vanishing rates indicates a stronger intermolecular interaction in HP-LLPS than in LP-LLPS, which might promote transformation into irreversible aggregates in the droplets. Further, direct transition from HP-LLPS to LP-LLPS was observed. This indicates that interconversion between LP-LLPS and HP-LLPS occurs in equilibrium. Formation of reversible liquid droplets, followed by phase transition into another liquid phase, could thus be part of the physiological maturation process of FUS-LLPS.
Topics & Concepts
ChemistryNucleationKineticsThermodynamicsCrystallographyOrganic chemistryQuantum mechanicsPhysicsRNA Research and SplicingRNA and protein synthesis mechanismsProtein Structure and Dynamics