Intelligent demethylase-driven DNAzyme sensor for highly reliable metal-ion imaging in living cells
Hong Chen, Qing Wang, Yingying Chen, Yuhui Gao, Jinhua Shang, Xiaocheng Weng, Xiaoqing Liu, Fuan Wang
Abstract
A methylation group that incapacitates the DNAzyme probe, thus eliminating possible off-site signal leakage, while the cell-specific demethylase-mediated removal of methylation modification could efficiently restore the initial catalytic DNAzyme for sensing metal ions, thus allowing a high-contrast bioimaging in live cells. This epigenetic repair-to-activate DNAzyme strategy may facilitate the robust visualization of disease-specific biomarkers for in-depth exploration of their biological functions.
Topics & Concepts
DeoxyribozymeDemethylaseNanotechnologyChemistryMetalBiophysicsBiochemistryMaterials scienceBiologyDNAOrganic chemistryHistoneAdvanced biosensing and bioanalysis techniquesTrace Elements in HealthRNA and protein synthesis mechanisms