UBC13-Mediated Ubiquitin Signaling Promotes Removal of Blocking Adducts from DNA Double-Strand Breaks
Remi Akagawa, Hai Thanh Trinh, Liton Kumar Saha, Masataka Tsuda, Kouji Hirota, Shintaro Yamada, Atsushi Shibata, Masato T. Kanemaki, Shinichiro Nakada, Shunichi Takeda, Hiroyuki Sasanuma
Abstract
by facilitating localization of RAP80 and BRCA1 to DSB sites and complex formation between BRCA1 and MRE11 at DSB sites. UBC13 and MRE11 are dispensable for restriction-enzyme-induced "clean" DSBs repair but responsible for over 50% and 70% of NHEJ-dependent repair of γ-ray-induced "dirty" DSBs, respectively. In conclusion, ubiquitin signaling promotes nucleolytic removal of DSB blocking adducts by MRE11 before NHEJ.
Topics & Concepts
DNANucleaseUbiquitinCell biologyHomologous recombinationDNA repairDNA damageChemistryNon-homologous end joiningTopoisomeraseBiochemistryBiologyMolecular biologyGeneDNA Repair MechanismsCancer therapeutics and mechanismsPolyomavirus and related diseases