Microbial consortia inoculation of woody legume Erythrina brucei increases nodulation and shoot nitrogen and phosphorus under greenhouse conditions
Belay Berza Beyene, Marcela C. Pagano, Prabavathy Vaiyapuri R, Fassil Assefa Tuji
Abstract
The legume-rhizobium symbiosis provides Nitrogen (N), while Legume-AMF symbiosis improves Phosphorus (P) supply to plants. This research was conducted to evaluate the symbiotic effectiveness of the Bradyrhizobium spp. and consortial inoculation of plant growth promoting bacteria -Bradyrhizobium shewense (AU27) and Acinetobacter soli (AU4), and arbuscular mycorrhizhal fungi Glomus sp.1 (AMF1) and Acaulospora sp.1 (AMF2), on growth, production and shoot N and P content of Erythrina brucei.The bacterial and mycorrhizal species were evaluated for phyto-beneficial properties in the greenhouse as individual as well as consortial inoculation.. All Bradyrhizobium species were effective for symbiotic nitrogen fixation. Consortial inoculations comprising of B. shewense (AU27) + A. soli (AU4) + Glomus sp.1 (AMF1) + Acaulospora sp.1 (AMF2) (T7) increased shoot length and shoot dry weight by 140% and 268%, respectively compared to un-inoculated control. Inoculations that involved B. shewense (AU27) + A. soli (AU4) increased shoot nitrogen by 260%, and 1200% increment of shoot P was recorded with inoculations of B. shewense (AU27) + Glomus sp.1 (AMF1) compared to un-inoculated control. These microbial inputs could be candidates for growth enhancement and shoot nitrogen and phosphorus improvement in Erythrina brucei and also as sustainable and eco-friendly agriculture input.