High-Level Production of Lysine in the Yeast Saccharomyces cerevisiae by Rational Design of Homocitrate Synthase
Shota Isogai, Tomonori Matsushita, H. Imanishi, Jirasin Koonthongkaew, Yoichi Toyokawa, Akira Nishimura, Xiao Yi, Romas J. Kazlauskas, Hiroshi Takagi
Abstract
A traditional method for screening toxic analogue-resistant mutants has been established for the breeding of microbes that produce high levels of amino acids, including lysine. However, another efficient strategy is required to further improve their productivity.
Topics & Concepts
Saccharomyces cerevisiaeLysineYeastRational designBiochemistryBiologyMutantProductivityProduction (economics)BiotechnologyAmino acidGeneGeneticsEconomicsMacroeconomicsFungal and yeast genetics researchMicrobial Metabolic Engineering and BioproductionEnzyme Structure and Function