Ac Biosusceptometry and Magnetic Nanoparticles to Assess Doxorubicin-Induced Kidney Injury in Rats
André G. Próspero, P.F. Oliveira, Guilherme Augusto Soares, Milena F Miranda, Leonardo Antonio Pinto, Daniela Carvalho dos Santos, Vanessa dos S. Silva, Nícholas Zufelato, Andris F. Bakuzis, José Ricardo de Arruda Miranda
Abstract
Aim: This paper aims to investigate a doxorubicin (DOX) chronic kidney disease rat model using magnetic nanoparticles (MNPs) associated with the alternate current biosusceptometry (ACB) to analyze its different perfusion profiles in both healthy and DOX-injured kidneys. Materials & methods: We used the ACB to detect the MNP kidney perfusion in vivo. Furthermore, we performed biochemical and histological analyses, which sustained results obtained from the ACB system. We also studied the MNP biodistribution. Results: We found that DOX kidney injury alters the MNPs' kidney perfusion. These changes became more intense as the disease progressed. Moreover, DOX has an important effect on MNP biodistribution as the disease evolved. Conclusion: This study provides new applications of MNPs in nephrology, instrumentation, pharmacology, physiology and nanomedicine.