An improved capillary isoelectric focusing-mass spectrometry method for high-resolution characterization of monoclonal antibody charge variants
Tian Xu, Linjie Han, Alayna M. George Thompson, Liangliang Sun
Abstract
We developed an automated cIEF-MS method with good stability and high resolution for characterizing charge variants of mAbs by bettering capillary neutral coating, reducing catholyte pH to 10, and optimizing the cIEF separation conditions.
Topics & Concepts
Isoelectric focusingChromatographyMass spectrometryResolution (logic)ChemistryCharacterization (materials science)Monoclonal antibodyAnalytical Chemistry (journal)High resolutionCapillary electrophoresisLow resolutionAntibodyMaterials scienceNanotechnologyBiologyComputer scienceBiochemistryRemote sensingArtificial intelligenceGeologyImmunologyEnzymeProtein purification and stabilityMass Spectrometry Techniques and ApplicationsMonoclonal and Polyclonal Antibodies Research