Specific Binding of a <scp>d</scp> ‐RNA G‐Quadruplex Structure with an <scp>l</scp> ‐RNA Aptamer
Chun‐Yin Chan, Chun Kit Kwok
Abstract
G-quadruplex (G4) structures are of general importance in chemistry and biology, such as in biosensing, gene regulation, and cancers. Although a large repertoire of G4-binding tools has been developed, no aptamer has been developed to interact with G4. Moreover, the G4 selectivity of current toolkits is very limited. Herein, we report the first l-RNA aptamer that targets a d-RNA G-quadruplex (rG4). Using TERRA rG4 as an example, our results reveal that this l-RNA aptamer, Ap3-7, folds into a unique secondary structure, exhibits high G4 selectivity and effectively interferes with TERRA-rG4-RHAU53 binding. Our approach and findings open a new door in further developing G4-specific tools for diverse applications.
Topics & Concepts
AptamerG-quadruplexRNANucleic acid structureRiboswitchChemistryComputational biologyBiologyGeneNon-coding RNAMolecular biologyGeneticsDNADNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms