The Manipulation of the Internal Hydrophobicity of FraC Nanopores Augments Peptide Capture and Recognition
Florian Leonardus Rudolfus Lucas, Kumar Sarthak, Erica Mariska Lenting, David Coltan, Nieck Jordy van der Heide, Roderick Corstiaan Abraham Versloot, Aleksei Aksimentiev, Giovanni Maglia
Abstract
ionic current blockades in FraC, and characterized the effect of the pore modification on peptide discrimination. This work provides insights to improve the recognition and to augment the capture of peptides by nanopores, which is important for developing a real-time and single-molecule size analyzer for peptide recognition and identification.
Topics & Concepts
NanoporePeptideMolecular recognitionAnalyteMolecular dynamicsBiophysicsNanotechnologyChemistryMoleculeAmino acidMaterials scienceBiochemistryBiologyComputational chemistryChromatographyOrganic chemistryNanopore and Nanochannel Transport StudiesIon-surface interactions and analysisMicrofluidic and Capillary Electrophoresis Applications