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Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells

Waleed M. Kholosy, Marieke Visscher, Kim Ogink, Helen Buttstedt, Kelli Griffin, Axel Beier, Jan P. Gerlach, Jan J. Molenaar, Niels Geijsen, Marco de Boer, Anna Chatsisvili

2021Journal of Biotechnology23 citationsDOI

Topics & Concepts

CRISPRCas9Genome editingElectroporationInduced pluripotent stem cellComputational biologyBiologyHEK 293 cellsEmbryonic stem cellCell biologyCell cultureGeneGeneticsCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchCAR-T cell therapy research
Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells | Litcius