Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis
Yuan‐I Chen, Yin-Jui Chang, Yuansheng Sun, Shih‐Chu Liao, Samantha R. Santacruz, Hsin‐Chih Yeh
Abstract
(fluorescence lifetime imaging based on a generative adversarial network). This work offers a new approach for STED imaging in situations where only a limited photon budget is available.
Topics & Concepts
STED microscopyFluorescenceImage resolutionSuperresolutionFluorescence-lifetime imaging microscopyMaterials sciencePhotonResolution (logic)OpticsStimulated emissionPhysicsArtificial intelligenceComputer scienceLaserImage (mathematics)Advanced Fluorescence Microscopy TechniquesAdvanced Electron Microscopy Techniques and ApplicationsIntegrated Circuits and Semiconductor Failure Analysis