SARS-CoV-2 epitope–specific CD4 <sup>+</sup> memory T cell responses across COVID-19 disease severity and antibody durability
Ryan Nelson, Yuezhou Chen, Olivia Venezia, Richard M. Majerus, Daniel S. Shin, MGH COVID-19 Collection & Processing Team, Mary Carrington, Xu G. Yu, Duane R. Wesemann, James J. Moon, Andrew D. Luster
Abstract
CD4 + T cells are central to long-term immunity against viruses through the functions of T helper 1 (T H 1) and T follicular helper (T FH ) cell subsets. To better understand the role of these subsets in coronavirus disease 2019 (COVID-19) immunity, we conducted a longitudinal study of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–specific CD4 + T cell and antibody responses in convalescent individuals who seroconverted during the first wave of the pandemic in Boston, MA, USA, across a range of COVID-19 disease severities. Analyses of spike (S) and nucleocapsid (N) epitope–specific CD4 + T cells using peptide and major histocompatibility complex class II (pMHCII) tetramers demonstrated expanded populations of T cells recognizing the different SARS-CoV-2 epitopes in most individuals compared with prepandemic controls. Individuals who experienced a milder disease course not requiring hospitalization had a greater percentage of circulating T FH (cT FH ) and T H 1 cells among SARS-CoV-2–specific cells. Analysis of SARS-CoV-2–specific CD4 + T cells responses in a subset of individuals with sustained anti-S antibody responses after viral clearance also revealed an increased proportion of memory cT FH cells. Our findings indicate that efficient early disease control also predicts favorable long-term adaptive immunity.