Antimutagenic bis (2-ethylhexyl) phthalate isolated from octopus (Paraoctopus vulgaris)
Susana-Gabriela Cruz-Ramírez, Carmen María López‐Saiz, Ema Carina Rosas‐Burgos, Francisco J. Cinco‐Moroyoqui, Carlos Velázquez, Javier Hernández, Armando Burgos‐Hernández
Abstract
In the present study, the antimutagenic activity of methanolic (MP) and hexanic (HP) phase from hexane-soluble extract of Paraoctopus vulgaris was evaluated. The HP showed significant concentration-dependent antimutagenic activity (p < 0.05) in the Ames assay against aflatoxin B1 (AFB1) using Salmonella typhimurium TA98 and TA100 tester strains. Seven fractions (F1-F7) were obtained from HP by silica gel-column chromatography and the antimutagenic activity of F6 and F7 caused significant reduction (p < 0.05) in the number of AFB1-induced revertants in both strains. However, high antimutagenic activity was exhibited in TA98 strain (85-97% reversion inhibition) and remained at a fraction-concentration of 3 μg/mL. F6 and F7 composition was analyzed by high-performance liquid chromatography/photodiode array, both fractions shared one peak at 9.42 min suggesting presence of the same compound. 1H-NMR and 13C-NMR analysis results indicate that the bioactive compound is bis (2-ethylhexyl) phthalate. Based on of these results bis (2-ethylhexyl) phthalate exhibit antimutagenic activity, which allows to consider it for further investigation as a potential chemopreventive compound.