Organic-Solvent-Tolerant Carboxylic Ester Hydrolases for Organic Synthesis
Alexander Bollinger, Rebecka Molitor, Stephan Thies, Rainhard Koch, Cristina Coscolín, Manuel Ferrer, Karl‐Erich Jaeger
Abstract
Major challenges hampering biotechnological applications of esterases include the requirement to accept nonnatural and chemically demanding substrates and the tolerance of the enzymes toward organic solvents which are often required to solubilize such substrates. We describe here a high-throughput screening strategy to identify novel organic-solvent-tolerant carboxylic ester hydrolases (CEs). Among these enzymes, CEs active against water-insoluble bulky substrates were identified. Our results thus contribute to fostering the identification and biotechnological application of CEs.
Topics & Concepts
Organic solventChemistryOrganic synthesisOrganic chemistrySolventCarboxylic acidBiochemistryChemical engineeringCatalysisEngineeringEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionCatalysis for Biomass Conversion