Candida glabrata Transcription Factor Rpn4 Mediates Fluconazole Resistance through Regulation of Ergosterol Biosynthesis and Plasma Membrane Permeability
Pedro Pais, Raquel Califórnia, Mónica Galocha, Romeu Viana, Mihaela Ola, Mafalda Cavalheiro, Azusa Takahashi‐Nakaguchi, Hiroji Chibana, Geraldine Butler, Miguel C. Teixeira
Abstract
The ability to acquire azole resistance is an emblematic trait of the fungal pathogen Candida glabrata . Understanding the molecular basis of azole resistance in this pathogen is crucial for designing more suitable therapeutic strategies. This study shows that the C. glabrata transcription factor (TF) CgRpn4 is a determinant of azole drug resistance. RNA sequencing during fluconazole exposure revealed that CgRpn4 regulates the expression of 212 genes, activating 80 genes and repressing, likely in an indirect fashion, 132 genes.
Topics & Concepts
ErgosterolCandida glabrataFluconazoleAzoleBiologyTranscription factorCell biologyMicrobiologyGeneBiochemistryAntifungalAntifungal resistance and susceptibilityFungal Biology and ApplicationsFungal and yeast genetics research