LIGHT-SABRE Hyperpolarizes 1-<sup>13</sup>C-Pyruvate Continuously without Magnetic Field Cycling
Andrey N. Pravdivtsev, Kai Buckenmaier, Nicolas Kempf, Gabriele Stevanato, Klaus Scheffler, J Engelmann, Markus Plaumann, Rainer Koerber, Jan‐Bernd Hövener, Thomas Theis
Abstract
High Resolution Image Download MS PowerPoint Slide Nuclear spin hyperpolarization enables real-time observation of metabolism and intermolecular interactions in vivo . 1- 13 C-pyruvate is the leading hyperpolarized tracer currently under evaluation in several clinical trials as a promising molecular imaging agent. Still, the quest for a simple, fast, and efficient hyperpolarization technique is ongoing. Here, we describe that continuous, weak irradiation in the audio-frequency range of the 13 C spin at the 121 μT magnetic field (approximately twice Earth’s field) enables spin order transfer from parahydrogen to 13 C magnetization of 1- 13 C-pyruvate. These so-called LIGHT-SABRE pulses couple nuclear spin states of parahydrogen and pyruvate via the J -coupling network of reversibly exchanging Ir-complexes. Using ∼100% parahydrogen at ambient pressure, we polarized 51 mM 1- 13 C-pyruvate in the presence of 5.1 mM Ir-complex continuously and repeatedly to a polarization of 1.1% averaged over free and catalyst-bound pyruvate. The experiments were conducted at −8 °C, where almost exclusively bound pyruvate was observed, corresponding to an estimated 11% polarization on bound pyruvate. The obtained hyperpolarization levels closely match those obtained via SABRE-SHEATH under otherwise identical conditions. The creation of three different types of spin orders was observed: transverse 13 C magnetization along the applied magnetic field, 13 C z -magnetization along the main field B 0, and 13 C– 1 H zz -spin order. With a superconducting quantum interference device (SQUID) for detection, we found that the generated spin orders result from 1 H– 13 C J -coupling interactions, which are not visible even with our narrow linewidth below 0.3 Hz and at −8 °C.