Different Routes of Protein Folding Contribute to Improved Protein Production in Saccharomyces cerevisiae
Qi Qi, Feiran Li, Rosemary Yu, Martin K. M. Engqvist, Verena Siewers, J. Fuchs, Jens Nielsen
Abstract
Protein folding plays an important role in protein maturation and secretion. In recombinant protein production, many studies have focused on the folding pathway to improve productivity. Here, we identified two different routes for improving protein production by yeast. We found that improving folding precision is a better strategy. Dysfunction of this process is also associated with several aberrant protein-associated human diseases. Here, our findings about the role of glucosidase Cwh41p in the precision control system and the characterization of the strain with a more precise folding process could contribute to the development of novel therapeutic strategies.