Assessment of antioxidant activity of pure graphene oxide (GO) and composite V2O5/GO using DPPH radical and H2O2 scavenging assays
Huma Sadiq, Hanan Sadiq, Amir Sohail, Abdul Basit, Naseem Akhtar, Kiran Batool, Soleiman Hisaindee, Lubna Asghar
Abstract
Abstract In this research, the Co precipitation method was utilized to synthesize a nanocomposite of vanadium oxide (V 2 O 5 ) and graphene oxide (GO). Pure GO was synthesized by the modified hammers method. Using a 1:2 ratio of GO and V 2 O 5 and heating in the oven at 70 °C leading to the formation of V 2 O 5 /GO nanocomposite. By grafting of GO on V 2 O 5 surface, a high range of graphene oxidation in V 2 O 5 /GO allowed for better reduction with V 2 O 5 metal-oxide. The scanning electron microscope (SEM) images and X-ray diffraction (XRD) spectra provide evidence of the distinct phase of graphene oxide formation. The antioxidant activity of V 2 O 5 /GO nanocomposite was conducted in two vitro assays, focusing on neutralization of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and H 2 O 2 radicals. The uniqueness of the nanocomposite was evident from its concentration-dependent antioxidant activities. Interestingly, the V 2 O 5 /GO samples has showed 60% antioxidant performance in the H 2 O 2 assays compared to its constituent.