Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres
Szilárd Szikora, Tibor Novák, Tamás Gajdos, Miklós Erdélyi, József Mihály
Abstract
flight muscle sarcomeres make them ideally suitable for single molecule localization microscopy and structure averaging. Our approach allowed us to determine the position of dozens of H-zone and Z-disk proteins with a quasi-molecular, ~5-10 nm localization precision. The protocol described below provides an easy and reproducible method to prepare individual myofibrils for dSTORM imaging. In addition, it includes an in-depth description of a custom made and freely available software toolbox to process and quantitatively analyze the raw localization data.
Topics & Concepts
SarcomereMyofibrilBiological systemMyosinResolution (logic)Process (computing)Nanoscopic scaleComputer scienceNanotechnologyBiophysicsMaterials scienceCrystallographyChemistryBiologyArtificial intelligenceCell biologyBiochemistryOperating systemMyocyteAdvanced Fluorescence Microscopy TechniquesForce Microscopy Techniques and ApplicationsAdvanced Electron Microscopy Techniques and Applications