Multishot tomography for high-resolution in situ subtomogram averaging
Sagar Khavnekar, William Wan, Parijat Majumder, Wojciech Wietrzyñski, Philipp S. Erdmann, Jürgen M. Plitzko
Abstract
Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET can be slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic acquisition scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, which yields sub-nanometer in situ STA averages.
Topics & Concepts
TomographyContext (archaeology)Cryo-electron tomographyResolution (logic)Tilt (camera)Tomographic reconstructionElectron tomographyOpticsFocused ion beamIn situBeam (structure)Materials sciencePhysicsComputer scienceArtificial intelligenceIonElectron microscopeGeologyEngineeringScanning transmission electron microscopyQuantum mechanicsPaleontologyMeteorologyMechanical engineeringAdvanced Electron Microscopy Techniques and ApplicationsElectron and X-Ray Spectroscopy TechniquesAdvanced X-ray Imaging Techniques