<i>Post-hoc</i> assessment of the immunogenicity of three antibodies reveals distinct immune stimulatory mechanisms
Robin E. Walsh, Megan B. Lannan, Yi Wen, Xiaoli Wang, Christopher A. Moreland, Jill A. Willency, Michael D. Knierman, Laura J. Spindler, Ling Liu, Wei Zeng, Guilherme V. Rocha, Victor H. Obungu, Jirong Lu, Arunan Kaliyaperumal, Andrea Ferrante, Robert W. Siegel, Laurent Malherbe
Abstract
. All three antibodies exhibited a moderate to high internalization by human DCs and MHC-associated peptide proteomics analysis revealed the presence of potential T cell epitopes that were confirmed by a T-cell proliferation assay. Collectively, these findings highlight the existence of distinct immune stimulatory mechanisms for immunogenic antibodies. These findings have implications for the preclinical immunogenicity risk assessment of biologics.
Topics & Concepts
ImmunogenicityImmune systemAntibodyT cellEpitopeImmunologyMonoclonal antibodyBiologyAntigenInnate immune systemPeripheral blood mononuclear cellIn vitroBiochemistryImmunotherapy and Immune ResponsesMonoclonal and Polyclonal Antibodies Researchvaccines and immunoinformatics approaches